Reporter

Part:BBa_J100104:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2012-12-13)


For Testing New Promoters via Golden Gate Assembly v2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1611
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1612
    Illegal PstI site found at 1626
    Illegal NotI site found at 7
    Illegal NotI site found at 1619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1612
    Illegal PstI site found at 1626
    Illegal AgeI site found at 47
    Illegal AgeI site found at 155
    Illegal AgeI site found at 1484
    Illegal AgeI site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834
    Illegal BsaI.rc site found at 28


Design Notes

J100104 contains BBa biobrick prefix + 4 base overhang + BsaI site cutting to the left + reverse complement of blue reporter protein Part:BBa_J100103 + BsaI site that cuts to the right + 4 base overhang + BD18 bicistronic RBS for more reliable translation (Part:BBa_J119024) + mRFP Part:BBa_E1010 + BBa biobrick suffix.



Source

Built from PCR of J100103 and J100091 on modified version of pSB1A2 that has its BsaI site removed from the AmpR gene.

References